A real-time spike classification method based on dynamic time warping for extracellular enteric neural recording with large waveform variability.

TitleA real-time spike classification method based on dynamic time warping for extracellular enteric neural recording with large waveform variability.
Publication TypeJournal Article
Year of Publication2016
AuthorsY Cao, N Rakhilin, PH Gordon, X Shen, and EC Kan
JournalJournal of Neuroscience Methods
Volume261
Start Page97
Pagination97 - 109
Date Published03/2016
Abstract

Computationally efficient spike recognition methods are required for real-time analysis of extracellular neural recordings. The enteric nervous system (ENS) is important to human health but less well-understood with few appropriate spike recognition algorithms due to large waveform variability.Here we present a method based on dynamic time warping (DTW) with high tolerance to variability in time and magnitude. Adaptive temporal gridding for "fastDTW" in similarity calculation significantly reduces the computational cost. The automated threshold selection allows for real-time classification for extracellular recordings.Our method is first evaluated on synthesized data at different noise levels, improving both classification accuracy and computational complexity over the conventional cross-correlation based template-matching method (CCTM) and PCA+k-means clustering without time warping. Our method is then applied to analyze the mouse enteric neural recording with mechanical and chemical stimuli. Successful classification of biphasic and monophasic spikes is achieved even when the spike variability is larger than millisecond in width and millivolt in magnitude.In comparison with conventional template matching and clustering methods, the fastDTW method is computationally efficient with high tolerance to waveform variability.We have developed an adaptive fastDTW algorithm for real-time spike classification of ENS recording with large waveform variability against colony motility, ambient changes and cellular heterogeneity.

DOI10.1016/j.jneumeth.2015.12.006
Short TitleJournal of Neuroscience Methods