A metabolic signature of colon cancer initiating cells.

TitleA metabolic signature of colon cancer initiating cells.
Publication TypeJournal Article
Year of Publication2014
AuthorsK-Y Chen, X Liu, P Bu, C-S Lin, N Rakhilin, JW Locasale, and X Shen
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume2014
Start Page4759
Pagination4759 - 4762
Date Published01/2014
Abstract

Colon cancer initiating cells (CCICs) are more tumorigenic and metastatic than the majority of colorectal cancer (CRC) cells. CCICs have also been associated with stem cell-like properties. However, there is a lack of system-level understanding of what mechanisms distinguish CCICs from common CRC cells. We compared the transcriptomes of CD133+ CCICs and CD133- CRC cells from multiple sources, which identified a distinct metabolic signature for CD133(high) CCICs. High-resolution unbiased metabolomics was then performed to validate this CCIC metabolic signature. Specifically, levels of enzymes and metabolites involved in glycolysis, the citric acid (TCA) cycle, and cysteine and methionine metabolism are altered in CCICs. Analyses of the alterations further suggest an epigenetic link. This metabolic signature provides mechanistic insights into CCIC phenotypes and may serve as potential biomarkers and therapeutic targets for future CRC treatment.

DOI10.1109/embc.2014.6944688
Short TitleAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings